The double advantage of Centrifugal Partition Chromatography is that solvent consumption and purification process time are drastically reduced.
We work to identify the appropriate solvent and the hydrodynamic characterization of each stationary phase in order to optimize purification methods at the industrial scale. We use digital models to help transfer the process to the industrial scale and to ensure that the projected calculations are valid.
Our engineering and R&D services
Our experts work with manufacturers of extracts, fragrances, biomolecules and active ingredients. Together, we optimize purification processes by developing and adjusting the processes and enriching and purifying molecules.
Molecule Extraction and Purification
Comparing extraction processes, purifying natural and synthetic substances, and developing methods for fractionating molecules of interest
High-performance liquid chromatography (HPLC)
CCD (Counter Current Distribution)
Fractionation of biotechnologically produced metabolites
Characterizing the extract before fractionation and selecting the appropriate separation method to obtain an optimal concentration of the purified substance
Evaluating complexity and researching the best separation principles
Capturing metabolites: utilizing liquid phases to recover metabolites at bioreactor outlets (photobioreactors, fermenters)
Concentration and purification: combining enrichment and concentration operations in accordance with specifications and process ecodesign
CPC column design, sizing, and characterization
Optimizing separation at a given scale, scaling up, or determining optimal dimensions of a centrifugal partition chromatography column for industrial-scale purification.
Approving the feasibility of commercial pilots and prototypes
Selecting solvents and the principle of separation: tests in pillboxes with targeted molecules, determining the physicochemical parameters of the separation system
Conducting research on solvent substitution and using green solvents
Hydrodynamics and mass transfer: identifying and lifting limitations, process intensification, sizing parameters
Defining the dimensions of a centrifugal partition chromatography column: optimizing productivity and seeking a techno-economic optimum
Purification process mastery and optimization
Reducing the number of steps in the purification process for natural substances with high added value (peptides, alkaloids)
Choice of two-phase liquid-liquid system: eluent and stationary phase
Assistance in choosing alternative solvents (biosolvent, green solvent) and ecodesign
Choice of separation technique: partition, displacement, ion exchange, chelation
Optimizing operating conditions
Preparing CPC column scale changes
Scaling a CPC purification process to the industrial scale and reducing the volume of solvent used by 75%.
Developing CPC column scale-up methodologies: approving phase hydrodynamics at different scales and verifying separation performances
Establishing specifications for separation and the technical specifications of equipment
Choosing equipment and an appropriate supplier
Approving the scale-up
Challenges we’ve met
Scaling up CPC purification
Interview with Laurent Petit, Minakem’s R&D Manager
Industrializing purification using centrifugal partition chromatography
Centrifugal Partition Chromatography (CPC) is known for its laboratory applications. Under certain conditions, it can also be used at the industrial scale. But changing the scale involves taking specific precautions and requires the skills of an experienced team. A pharmaceutical laboratory recognized CAPACITÉS SAS’ experts’ knowledge in the field of CPC. The lab asked them to determine and approve conditions for the industrial-level scaling o…