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Centrifugal Partition Chromatography

The double advantage of Centrifugal Partition Chromatography is that solvent consumption and purification process time are drastically reduced.

We work to identify the appropriate solvent and the hydrodynamic characterization of each stationary phase in order to optimize purification methods at the industrial scale. We use digital models to help transfer the process to the industrial scale and to ensure that the projected calculations are valid.

Our engineering and R&D services

Our experts work with manufacturers of extracts, fragrances, biomolecules and active ingredients. Together, we optimize purification processes by developing and adjusting the processes and enriching and purifying molecules.

Molecule Extraction and Purification

Comparing extraction processes, purifying natural and synthetic substances, and developing methods for fractionating molecules of interest

  • Reactive extraction
  • Distillation
  • Membrane filtration
  • High-performance liquid chromatography (HPLC)
  • CCD (Counter Current Distribution)
  • Flash chromatography

Fractionation of biotechnologically produced metabolites

Characterizing the extract before fractionation and selecting the appropriate separation method to obtain an optimal concentration of the purified substance

  • Evaluating complexity and researching the best separation principles
  • Capturing metabolites: utilizing liquid phases to recover metabolites at bioreactor outlets (photobioreactors, fermenters)
  • Concentration and purification: combining enrichment and concentration operations in accordance with specifications and process ecodesign

CPC column design, sizing, and characterization

Optimizing separation at a given scale, scaling up, or determining optimal dimensions of a centrifugal partition chromatography column for industrial-scale purification.

  • Approving the feasibility of commercial pilots and prototypes
  • Selecting solvents and the principle of separation: tests in pillboxes with targeted molecules, determining the physicochemical parameters of the separation system
  • Conducting research on solvent substitution and using green solvents
  • Hydrodynamics and mass transfer: identifying and lifting limitations, process intensification, sizing parameters
  • Defining the dimensions of a centrifugal partition chromatography column: optimizing productivity and seeking a techno-economic optimum

Purification process mastery and optimization

Reducing the number of steps in the purification process for natural substances with high added value (peptides, alkaloids)

  • Choice of two-phase liquid-liquid system: eluent and stationary phase
  • Assistance in choosing alternative solvents (biosolvent, green solvent) and ecodesign
  • Choice of separation technique: partition, displacement, ion exchange, chelation
  • Optimizing operating conditions

Preparing CPC column scale changes

Scaling a CPC purification process to the industrial scale and reducing the volume of solvent used by 75%.

  • Developing CPC column scale-up methodologies: approving phase hydrodynamics at different scales and verifying separation performances
  • Establishing specifications for separation and the technical specifications of equipment
  • Choosing equipment and an appropriate supplier
  • Approving the scale-up

Challenges we’ve met

Scaling up CPC purification

Interview with Laurent Petit, Minakem’s R&D Manager

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Purifying an active substance for the perfume industry

Gilson chose Capacités to prepare an active substance intended for the perfume industry.

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Industrializing purification using centrifugal partition chromatography

Centrifugal Partition Chromatography (CPC) is known for its laboratory applications. Under certain conditions, it can also be used at the industrial scale. But changing the scale involves taking specific precautions and requires the skills of an experienced team. A pharmaceutical laboratory recognized CAPACITÉS SAS’ experts’ knowledge in the field of CPC. The lab asked them to determine and approve conditions for the industrial-level scaling o…

Read this success story
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Need an innovative and resourceful partner?

Capacités teams inspire, harness and couple numerous cutting-edge skills covering a wide range of applications and expertise to meet all of your innovation needs with passion and commitment!

+33 (0)2 72 64 88 94

or send us a message:





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Need an innovative and resourceful partner?

Capacités teams inspire, harness and couple numerous cutting-edge skills covering a wide range of applications and expertise to meet all of your innovation needs with passion and commitment!

+33 (0)2 72 64 88 94

or send us a message:





Required fields

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