Our Capacités experts in analytical chemistry have developed an exceptional know-how in Nuclear Magnetic Resonance (1D NMR and 2D NMR) and in Isotope Ratio Mass Spectrometry (IRMS).
Access to a large pool of equipment allows us to support industrial projects, from sample preparation to characterization.
We are specialized in very high precision quantification projects and in the analysis of complex matrices. We help our clients to innovate in the study of metabolism, the fight against counterfeiting, the determination of the purity of active molecules, or the monitoring of chemical transformations.
Summary
Services
Strategy and consulting
Diagnosis and selection
Process development
Quality control
Development of methods
Validate a therapeutic target or refine the description of a pathology through the identification and qualification of biomarkers responsible for changes between two states.
Our experts master different types of analyses of biological samples. They perform targeted and non-targeted profiling and highlight changes between states of the biological matrices studied.
In a nutshell
Targeted analysis
Metabolomic profiling
Metabolic Footprint
Analysis of matrices
Data processing
Our expertise is to qualify reference substances, determine the purity of molecules and certify their compliance with international quality standards.
Our experts assist pharmaceutical and cosmetic companies in certifying the purity of their reference molecules using NMR methods.
In a nutshell
NMR acquisition parameters
NMR processing parameters
Method validation and parameter verification
Product origin authentification
Optimization of the NMR tool
Didier G. Diomande, Estelle Martineau, Alexis Gilbert, Pierrick Nun, Ariaki Murata, Keita Yamada, Naoharu Watanabe, Illa Tea, Richard J. Robins, Naohiro Yoshida, and Gérald S. Remaud Position-Specific Isotope Analysis of Xanthines: A 13C Nuclear Magnetic Resonance Method to Determine the 13C Intramolecular Composition at Natural Abundance.v
Analytical Chemistry 2015 87 (13), 6600-6606. DOI: 10.1021/acs.analchem.5b00559
Development of fast and quantitative 2D NMR acquisition methods.
Our experts have developed a protocol for the implementation of targeted and non-targeted metabolic and lipidomic studies.
In a nutshell
Rapid quantification for metabolomics and lipidomics
Quantification of polysaccharides by 2D NMR
Estelle Martineau, Serge Akoka, Renaud Boisseau, Benoît Delanoue, and Patrick Giraudeau. Fast Quantitative 1H–13C Two-Dimensional NMR with Very High Precision. Analytical Chemistry 2013 85 (9), 4777-4783
Estelle Martineau, Kamel El Khantache, Marion Pupier, Patricia Sepulcri, Serge Akoka, Patrick Giraudeau, Non-linear effects in quantitative 2D NMR of polysaccharides: Pitfalls and how to avoid them, Journal of Pharmaceutical and Biomedical Analysis, Volume 108, 2015, Pages 78-85, ISSN 0731-7085
Estelle Martineau, Jean-Nicolas Dumez, Patrick Giraudeau. Fast quantitative 2D NMR for metabolomics and lipidomics: A tutorial Magnetic Resonance in Chemistry. 04 June 2019
To analyze the 13C, 15N, 2H, 18O and 34S isotopes present in organic matter, for a better understanding of metabolism in the fields of health and nutrition.
The mastery of compound-specific analysis allows to obtain 13C and/or 15N profiles of different matrices.
In a nutshell
Average isotopic composition by EA-IRMS
GC-IRMS analyse
Tea, I., Martineau, E., Antheaume, I. et al. 13C and 15N natural isotope abundance reflects breast cancer cell metabolism. Sci Rep 6, 34251 (2016). https://doi.org/10.1038/srep34251
Olivier L Mantha, Caroline Goupille, Jean-François Dumas, Richard Robins, Philippe Bougnoux, Régis Hankard, Arnaud De Luca, Natural isotopic abundances as markers of compliance in clinical trials, The American Journal of Clinical Nutrition, Volume 111, Issue 5, May 2020, Pages 1109–1110
Tea I, Le Guennec A, Frasquet-Darrieux M, Julien M, Romek K, Antheaume I, Hankard R, Robins RJ. Simultaneous determination of natural-abundance δ15N values and quantities of individual amino acids in proteins from milk of lactating women and from infant hair using gas chromatography/isotope ratio mass spectrometry. Rapid Commun Mass Spectrom. 2013 Jun 30;27(12):1345-53.
Our Analytical Chemistry experts perform their engineering duties in the very research laboratories of Nantes Université themselves, most notably at CEISAM. They strive to combine technical resources and scientific discovery to meet your R&D needs.
Challenges we’ve met
Quantification of polysaccharides by 2D NMR
Capacités teams inspire, harness and couple numerous cutting-edge skills covering a wide range of applications and expertise to meet all of your innovation needs with passion and commitment!
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